OligoCalculator was written by eugen buehler and modified by lab314.com
La inclusión de OligoCalculator en este sitio web ha sido autorizada expresamente por su autor
A Useful Hint From Bharat Patel's Laboratory
The OligoCalculator calculates Tm based on 0.195M monovalent cation (salt) concentration equal to 1x SSC. For calculating Tm values of oligonucleotides (10 to 50) at different monovalent cation concentrations, the formula given below is used. Using the Oligocalculator and the formula, you can calculate the Tm of your favourite oligonucleotide amplification primers eg in the Promega PCR buffer (which contains 0.05M monovalent cations) or the Tm of your DIG-labelled oligonucleotide probes which are usually used with 0.975M monovalent cations (5x SSC).
lab314.com tiene otros programas para cálculo de Peso Molecular de DNA, RNA y Proteínas.
RNA cadena sencilla y formato standard ( A, G, C, U )
RNA cadena doble y formato standard ( A, G, C, U )
RNA cadena sencilla y formato Fasta ( A, G, C, T )
RNA cadena doble y formato Fasta ( A, G, C, T )
Proteínas con aminoácidos en formato de una letra
Proteínas con aminoácidos en formato tres letras